Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Area of Science:

• Natural Product Chemistry
• Pharmacognosy
• Analytical Chemistry

Background:

• Natural products are a vital source for novel drug discovery.
• Phenolic compounds, abundant in nature, exhibit significant antioxidant activities.
• Effective isolation of bioactive compounds is crucial for drug development.

Purpose of the Study:

• To review various chromatographic and advanced techniques for isolating antioxidant phenolic compounds.
• To highlight the advantages and limitations of different separation methodologies.
• To identify promising methods for efficient purification of natural phenolic antioxidants.

Main Methods:

• Column Chromatography (CC)
• Medium-Pressure Liquid Chromatography (MPLC)
• High-Performance Liquid Chromatography (HPLC)
• Counter-Current Chromatography (CCC)
• Hydrophilic Interaction Chromatography (HILIC)
• Supercritical Fluid Chromatography (SFC)
• Molecularly Imprinted Technologies (MIT)
• High-Performance Thin Layer Chromatography (HPTLC)

Main Results:

• Various chromatographic techniques effectively separate phenolic compounds.
• Molecularly Imprinted Technologies (MIT) provide a direct and efficient purification route.
• MIT reduces complexity in the isolation process, offering higher accessibility.
• Further refinement and development of MIT are needed for broader application.

Conclusions:

• Chromatographic methods are essential for isolating antioxidant phenolic compounds.
• Molecularly Imprinted Technologies show significant promise for efficient and direct purification.
• Continued research into MIT will enhance its utility in natural product drug discovery.