Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Therapy and biomarker dependent progression-free survival in infant sonic hedgehog medulloblastoma: a multi-national retrospective cohort study.

EClinicalMedicine·2026

Same author

Genetic discrimination in workplace, insurance industry and legal system: A review.

Legal medicine (Tokyo, Japan)·2026

Same author

Lewy bodies are surrounded by granulovacuolar degeneration bodies in dementia with Lewy bodies.

Brain pathology (Zurich, Switzerland)·2026

Same author

The seminal proteome of a monandrous fly, Drosophila subobscura.

Proceedings. Biological sciences·2026

Same author

Integrated biomarker and treatment correlates of prognosis in infant non-WNT/non-SHH medulloblastoma: a multinational retrospective cohort study.

The Lancet. Child & adolescent health·2026

Same author

Simulating Compassion: Enhancing Graduate Nurses' Preparedness for End-of-Life and Palliative Care.

Nursing education perspectives·2026

Same journal

Identification of Age-Associated Circulating Proteins and Lipids in 3800 Comorbidity-Enriched Older Adults from Japan-Based Cohorts Using Olink Assays and MRM Mass Spectrometry.

Journal of proteome research·2026

Same journal

Molecular Solution to the Paradox of Ancient Brain Preservation.

Journal of proteome research·2026

Same journal

From Method-Defined Signals to Reference Measurement Procedures: Two Decades of Mass Spectrometry-Based ProGRP Quantification.

Journal of proteome research·2026

Same journal

Proteomic Profiling of Extracellular Vesicle-Enriched Plasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal Adenocarcinoma.

Journal of proteome research·2026

Same journal

Computationally Efficient Bayesian Estimation of Graphical Networks for Omics Data.

Journal of proteome research·2026

Same journal

Hierarchy of MS-Based Evidence.

Journal of proteome research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 28, 2025

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

Bone Proteomics Method Optimization for Forensic Investigations.

Luke Gent¹, Maria Elena Chiappetta^1,2, Stuart Hesketh³

¹School of Law and Policing, Research Centre for Field Archaeology and Forensic Taphonomy, University of Central Lancashire, Preston PR1 2HE, United Kingdom.

Journal of Proteome Research

|April 15, 2024

Summary

This summary is machine-generated.

This study optimized proteomic analysis of bone for forensic science. The S-Trap method and data-independent acquisition (DIA) improved protein identification and reproducibility in skeletal remains.

Keywords:

acquisition modes bone proteomics forensic science mass spectrometry protein extraction

More Related Videos

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts

Published on: January 29, 2018

Skeletal Muscle Gender Dimorphism from Proteomics

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

Related Experiment Videos

Last Updated: Jun 28, 2025

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts

Published on: January 29, 2018

Skeletal Muscle Gender Dimorphism from Proteomics

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

Area of Science:

Forensic proteomics
Biomolecular analysis
Mass spectrometry

Background:

Proteomic analysis of skeletal remains aids forensic investigations.
Maximizing protein recovery and minimizing laboratory errors are crucial for forensic casework.
Standardization of proteomic workflows is needed for reliable results.

Purpose of the Study:

To compare different bone protein extraction and analysis workflows for forensic applications.
To evaluate the impact of novel S-Trap technology and data acquisition methods (DDA vs. DIA) on proteomic outcomes.
To enhance the reproducibility and sensitivity of proteomic analysis in forensic bone samples.

Main Methods:

Comparison of in-StageTip, S-Trap with lysis solution 1, and S-Trap with lysis solution 2 workflows.
Analysis of 30 human cortical tibiae samples using liquid chromatography with tandem mass spectrometry (LC-MS/MS).
Evaluation of data-dependent acquisition (DDA) versus data-independent acquisition (DIA) for protein identification.

Main Results:

S-Trap workflows yielded higher proteome recovery and reduced induced deamidations compared to in-StageTip.
Data-independent acquisition (DIA) demonstrated greater sensitivity and broader protein identification, especially for low-abundance proteins.
Open-source software enhanced protein identification in both DDA and DIA modes.

Conclusions:

The S-Trap protocol is well-suited for forensic bone proteomic analysis.
Combining S-Trap with DIA offers improved proteomic outcomes and reproducibility for forensic applications.
This optimized workflow contributes to the standardization of bone proteomic analyses in forensic science.