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Updated: Jun 28, 2025

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Preoperative oral hygiene treatment reduces bacterial transport and colonization during intubation for orthopedic
Yu Shuai1, Xin Wang1, Songli Chen2
1Department of Anesthesiology, The Second Affiliated Hospital of Zunyi Medical University.
Purpose:
The process of infection by bacteria and viruses involves invasion, establishment, growth, and parasitization. Poor oral hygiene and dysbiosis are significant risk factors for pneumonia. The aim of this study was to evaluate bacterial transport into the trachea during intubation for orthopedic surgery and the impact of oral hygiene treatment.
Methods:
The study cohort included 53 patients with fracture who underwent surgical procedures under general anesthesia and were divided into two groups: an oral hygiene treatment (OHT) group (n = 27) and a control group (n = 26). Before intubation, the OHT group underwent preoperative oral hygiene treatment. Microbiological culture was used for detection and counting of bacteria from the oropharynx, trachea, and tip of the endotracheal tube (ETT).
Results:
Patients in the OHT group had a lower pathogen detection rate and lower degree of bacterial colonization in the oropharynx, trachea, and ETT tip.
Conclusion:
Preoperative oral hygiene treatment is able to reduce bacterial transport and colonization during orthopedic surgery, thus providing an important adjunct to pre-anesthesia care.
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