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User-friendly oblique plane microscopy on a fully functional commercially available microscope base.

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This study introduces an oblique plane microscope (OPM) using a two-mirror scanning system. This design maintains image quality despite objective lens movement, enhancing OPM versatility.

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Area of Science:

  • Optical microscopy
  • Biomedical engineering

Background:

  • Commercial microscope bases offer advanced functionality but often have movable objective lenses.
  • Traditional oblique plane microscopes (OPMs) may face challenges with such mobile objectives.

Purpose of the Study:

  • To develop an OPM system compatible with commercial microscope bases.
  • To address the challenge of objective lens displacement in OPMs.

Main Methods:

  • Designed an OPM system with a two-mirror scanning geometry.
  • Integrated the OPM with a commercially available microscope base.
  • Tested performance with a 100X, 1.35 numerical aperture objective lens within a ±1 mm displacement range.

Main Results:

  • The two-mirror system compensated for objective lens displacement.
  • Point spread function (PSF) size increased by <3% (lateral) and <11% (axial) with ±1 mm displacement.
  • Magnification error remained below 0.5% within ±10 µm of the focal plane.

Conclusions:

  • The developed OPM system demonstrates robust performance with mobile objective lenses.
  • The two-mirror scanning geometry offers advantages in light efficiency and footprint compared to scan-lens/galvo-mirror systems.
  • This approach enhances the adaptability of OPMs for various microscopy platforms.