Maternal factor Trim75 contributes to zygotic genome activation program in mouse early embryos

Area of Science:

• Developmental Biology
• Genomics
• Epigenetics

Background:

• Zygotic genome activation (ZGA) is critical for early embryo development.
• Failure in ZGA is linked to human embryo developmental arrest.
• Maternal factors influencing ZGA require further investigation.

Purpose of the Study:

• To identify maternal factors driving Zygotic Genome Activation (ZGA).
• To elucidate mechanisms of ZGA in mammalian preimplantation embryos.

Main Methods:

• Utilized single-cell RNA sequencing data.
• Induced a 2-cell-like transition state in mouse embryonic stem cells.
• Employed RNA velocity analysis to identify potential gene drivers.

Main Results:

• Identified potential candidate markers for 2-cell-like cells.
• Overlap analysis suggested Trim75 as a potential driver of minor ZGA.
• Trim75 may recruit EP300 and establish H3K27ac in gene bodies.

Conclusions:

• Trim75 is a potential driver of minor ZGA.
• Trim75's role in recruiting EP300 and establishing H3K27ac is proposed.
• These mechanisms contribute to mammalian preimplantation embryo development.