Acute Neuropixels Recordings in the Marmoset Monkey.

Area of Science:

• Neuroscience
• Computational Neuroscience
• Primate Research

Background:

• High-density probes like Neuropixels offer insights into neural population activity within cortical layers.
• Marmoset monkeys' smooth cortex is ideal for perpendicular recordings to study laminar computations.

Purpose of the Study:

• To present a novel method for acute Neuropixels recordings in the common marmoset (Callithrix jacchus).
• To facilitate detailed investigation of neural activity across cortical layers in a primate model.

Main Methods:

• Developed an artificial silicon-based dura to replace the native dura, allowing visual access and perpendicular probe insertion.
• Chamber design is infection-resistant and compatible with semi-chronic microdrives and wireless recording hardware.
• Technique supports repeated acute penetrations over months, potentially up to a year with maintenance.

Main Results:

• Successfully enabled acute Neuropixels recordings in marmosets.
• The method ensures perpendicular cortical penetrations, avoiding blood vessels.
• Allows recording from hundreds of single neurons distributed throughout cortical columns.

Conclusions:

• This method provides a robust approach for high-density neural recordings in marmosets.
• It advances the study of laminar computations and neural population dynamics in primates.
• Facilitates long-term, detailed neural recordings for behavioral studies.