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Updated: Jun 27, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

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Published on: February 9, 2024

Engineering Gene-Specific DNAzymes for Accessible and Multiplexed Nucleic Acid Testing.

Lu Gao¹, Ke Yi², Yun Tan¹

¹Key Laboratory of Green Chemistry & Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.

|April 26, 2024

Summary

This summary is machine-generated.

We developed CLARISSA, a CRISPR-like assay using DNAzymes for sensitive point-of-care detection of genetic markers like human papillomavirus (HPV). This versatile tool offers accurate disease diagnosis in the field.

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Area of Science:

Molecular Biology
Biotechnology
Diagnostics

Background:

Accurate point-of-care detection of disease biomarkers is crucial for treatment and surveillance.
Existing methods may lack sensitivity, specificity, or field-deployability.

Purpose of the Study:

To develop and validate a novel nucleic acid detection assay, CLARISSA, for point-of-care applications.
To engineer RNA-cleaving DNAzymes for specific genetic marker detection and signal amplification.

Main Methods:

Engineered gene-specific DNAzymes (gDzs) for target recognition and cleavage.
Developed CLARISSA assay combining gDzs with isothermal amplification (recombinase polymerase amplification).
Validated CLARISSA for human papillomavirus (HPV) 16 detection in clinical cervical samples and multiplexed HPV16/18 detection.

Main Results:

CLARISSA achieved 100% sensitivity and 97.4% specificity for HPV16 detection in 189 samples.
Multiplexed CLARISSA showed 96.3% sensitivity for HPV16 and 83.3% for HPV18 in 46 samples.
Assay demonstrated compatibility with both fluorescence and lateral flow readouts, with no false positives.

Conclusions:

CLARISSA is a versatile, sensitive, and specific nucleic acid detection platform.
The assay is suitable for point-of-care and field-based clinical diagnosis.
CLARISSA presents a promising alternative to CRISPR-Dx for diverse diagnostic applications.