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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 27, 2025

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

TRBC1 in flow cytometry: Assay development, validation, and reporting considerations.

Katherine A Devitt^1,2, Wolfgang Kern³, Weijie Li⁴

¹Department of Pathology and Laboratory Medicine, University of Vermont Medical Center, Burlington, Vermont, USA.

Cytometry. Part B, Clinical Cytometry

|May 3, 2024

Summary

This summary is machine-generated.

The T-cell receptor constant region 1 (TRBC1) marker significantly improves T-cell neoplasm diagnosis in clinical flow cytometry. This study guides its integration into existing panels and interpretation for accurate T-cell clonality assessment.

Keywords:

T‐cell flow cytometry leukemia lymphoma validation

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Last Updated: Jun 27, 2025

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

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Published on: August 15, 2013

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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

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Published on: September 16, 2012

Area of Science:

Immunology
Hematopathology
Clinical Cytometry

Background:

T-cell clonality assessment via flow cytometry traditionally relies on suboptimal methods like aberrant marker expression.
Accurate diagnosis of T-cell neoplasms is crucial for effective patient treatment and management.

Purpose of the Study:

To introduce the T-cell receptor constant region 1 (TRBC1) as a promising marker for T-cell neoplasm diagnosis.
To provide guidance on incorporating TRBC1 into existing flow cytometry panels for T-cell workups.
To offer case examples and highlight potential interpretation challenges for routine clinical application.

Main Methods:

Review of TRBC1 marker properties and its application in flow cytometry.
Development and presentation of strategies for integrating TRBC1 into established T-cell panels.
Analysis of case studies illustrating TRBC1 utility and diagnostic considerations.

Main Results:

TRBC1 demonstrates significant potential to enhance the diagnostic accuracy of T-cell neoplasms.
Integration into existing panels is feasible, with practical examples provided.
Specific diagnostic scenarios requiring cautious interpretation of TRBC1 results are identified.

Conclusions:

TRBC1 is a valuable tool for improving T-cell clonality assessment in clinical flow cytometry.
Proper integration and interpretation are key to maximizing its diagnostic utility.
Further familiarization with TRBC1 is recommended for the flow cytometry community.