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Related Experiment Video

Updated: Jun 27, 2025

Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

Published on: May 31, 2022

Forward programming human pluripotent stem cells into microglia.

Júlia Csatári¹, Heinz Wiendl¹, Matthias Pawlowski¹

¹Department of Neurology with Institute of Translational Neurology, University Hospital Münster, Albert-Schweitzer-Campus 1, Building A1, 48149 Münster, Germany.

Trends in Cell Biology

|May 3, 2024

Summary

This summary is machine-generated.

Forward programming offers a faster, more efficient method for generating human microglia from stem cells. This review assesses protocols using transcription factors to optimize microglia production for research.

Keywords:

cell engineering differentiation genome engineering microglia reprogramming stem cells transcription factors

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Derivation of a Human Brain Organoid with Microglia Development

Derivation of a Human Brain Organoid with Microglia Development

Published on: January 17, 2025

Area of Science:

Neuroscience
Stem Cell Biology
Immunology

Background:

Microglia are crucial for central nervous system development, homeostasis, and defense.
Human induced pluripotent stem cells (hiPSCs) are a valuable in vitro model for microglia research.
Traditional hiPSC differentiation methods are slow, inconsistent, and inefficient.

Purpose of the Study:

To review and assess forward programming protocols for human microglia generation.
To analyze the impact of transcription factors, delivery methods, and media on microglia induction.
To identify optimal strategies for efficient, large-scale human microglia production.

Main Methods:

Comprehensive literature review of published forward programming protocols.
Analysis of protocols based on forced expression of key lineage transcription factors (TFs).
Evaluation of reprogramming factors, transgene delivery techniques, and medium compositions.

Main Results:

Forward programming emerges as a promising alternative for bulk human microglia generation.
Key factors influencing induction kinetics and microglia phenotype include TF choice, delivery, and media.
Optimized protocols can overcome limitations of traditional differentiation methods.

Conclusions:

Forward programming provides an efficient route to generate human microglia from hiPSCs.
Understanding the impact of specific protocol components is key to optimizing microglia production.
This approach facilitates robust in vitro studies of human microglia function and disease.