Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2025

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach
07:16

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach

Published on: April 25, 2025

150

Imaging Cell Signaling in Tissues Using the IBEX Method.

Yuxuan Meng1, Changtai Li1, Gregory S Patient1

  • 1UCL Institute of Ophthalmology, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2024
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

10-year experience in the use of Intravenous Immunoglobulin for Autoimmune Retinopathy.

Eye (London, England)·2026
Same author

The IBEX knowledge-base a community resource enabling adoption and development of immunofluorescence imaging methods.

eLife·2026
Same author

Mechanical and Electrical Properties dataset of Cu-Cr-X alloys generated with automated figure data extraction.

Scientific data·2025
Same author

Rebalancing viral and immune damage versus repair prevents death from lethal influenza infection.

Science (New York, N.Y.)·2025
Same author

Development of the advised protocol for OCT study terminology and elements anterior segment OCT extension reporting guidelines (APOSTEL-AS): Study protocol.

PloS one·2025
Same author

Leucine-rich α-2-glycoprotein 1 initiates the onset of diabetic retinopathy in mice.

Science translational medicine·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Highly multiplexed immunohistochemistry, like the innovative IBEX method, improves cell signaling studies by mapping protein locations in tissues. This protocol simplifies spatial biology research for better understanding tissue interactions.

Area of Science:

  • Tissue biology and spatial omics
  • Cellular signaling and molecular interactions

Background:

  • Understanding cell signaling in tissues requires precise localization of multiple proteins simultaneously.
  • Existing methods may lack the multiplexing capacity or simplicity for comprehensive spatial analysis.
  • Integrating protein data with tissue context (stroma, adjacent structures) is crucial for biological insights.

Purpose of the Study:

  • To present a simplified protocol for the In-situ Biodistribution by Exchange (IBEX) method for highly multiplexed immunohistochemistry on tissues.
  • To demonstrate the utility of IBEX for simultaneously localizing numerous cell signaling pathways within a tissue microenvironment.
  • To provide guidance for applying IBEX, showcasing its potential for spatial biology research.

Main Methods:

Keywords:
Cell signalingIBEXImmunofluorescenceImmunohistochemistryRetina

More Related Videos

Guidelines and Experience Using Imaging Biomarker Explorer IBEX for Radiomics
10:17

Guidelines and Experience Using Imaging Biomarker Explorer IBEX for Radiomics

Published on: January 8, 2018

13.2K
Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry
07:58

Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry

Published on: April 3, 2019

6.7K

Related Experiment Videos

Last Updated: Jun 27, 2025

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach
07:16

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach

Published on: April 25, 2025

150
Guidelines and Experience Using Imaging Biomarker Explorer IBEX for Radiomics
10:17

Guidelines and Experience Using Imaging Biomarker Explorer IBEX for Radiomics

Published on: January 8, 2018

13.2K
Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry
07:58

Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry

Published on: April 3, 2019

6.7K
  • Adaptation and simplification of the IBEX (In-situ Biodistribution by Exchange) cyclic immunohistochemistry protocol for tissue analysis.
  • Application of a 12-marker antibody panel for simultaneous detection of multiple proteins.
  • Utilizing human retina tissue as a model system to validate the protocol's effectiveness and demonstrate spatial protein distribution.

    • Main Results:

    • Successful implementation of a simplified IBEX protocol for highly multiplexed immunohistochemistry on tissue samples.
    • Demonstrated simultaneous localization of 12 different protein markers within the human retina.
    • Provided a clear visualization of protein spatial distribution and cellular context within the tissue.

    Conclusions:

    • The simplified IBEX method offers an accessible and powerful approach for high-plex spatial profiling in tissues.
    • This technique enhances the study of cell signaling by revealing the spatial organization of molecular pathways.
    • IBEX facilitates a deeper understanding of tissue architecture, cell interactions, and disease mechanisms through spatial omics.