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Comparison of Rodent Infectious Agent Detection by Exhaust Dust Testing and Traditional Sentinel Testing Using

Taylor Simmons1, Yesen Zhou1, Lea Ann Chlebek1

  • 1Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan.

Journal of the American Association for Laboratory Animal Science : JAALAS
|May 7, 2024
PubMed
Summary
This summary is machine-generated.

Exhaust dust testing (EDT) offers a promising alternative to soiled bedding sentinels (SBS) for rodent health surveillance. EDT demonstrates comparable or superior detection of common agents, reducing reliance on animal sentinels.

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Area of Science:

  • Veterinary diagnostics
  • Infectious disease surveillance
  • Laboratory animal science

Background:

  • Traditional rodent health surveillance relies on soiled bedding sentinels (SBS).
  • There is a growing need for improved diagnostic methods and reduced animal use in sentinel programs.
  • PCR-based methods like exhaust dust testing (EDT) are being explored for routine health monitoring.

Purpose of the Study:

  • To compare the efficacy of exhaust dust testing (EDT) with soiled bedding sentinels (SBS) for rodent health surveillance.
  • To evaluate the performance of EDT in detecting a panel of 19 infectious agents.
  • To assess the correlation between PCR-based detection and organism load.

Main Methods:

  • Comparison of absolute and quantitative PCR results from EDT filters and SBS mice.
  • Screening for 19 infectious agents across three facilities and multiple IVC racks.
  • Analysis of PCR copy numbers to estimate organism load and compare with cage census data.

Main Results:

  • EDT consistently detected *Helicobacter* spp. and *Corynebacterium bovis* more effectively than SBS.
  • EDT and SBS showed agreement for detecting murine norovirus (MNV).
  • EDT detected fewer cases of RCHPV-1 compared to SBS, indicating potential differences in sensitivity for specific agents.

Conclusions:

  • Exhaust dust testing (EDT) shows comparable or superior performance to soiled bedding sentinels (SBS) for detecting many common rodent pathogens.
  • EDT offers a viable alternative for routine rodent health surveillance, potentially reducing the need for animal sentinels.
  • Quantitative PCR data from EDT can provide insights into organism load and distribution within a colony.