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Sensing antibody functions with a novel CCR8-responsive engineered cell.

Jianyu Hao¹, Yitong Lv¹, Xufeng Xiao²

¹College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.

Acta Biochimica Polonica

|May 9, 2024

Summary

A new cell line aids in evaluating anti-CCR8 antibody therapies for cancer and autoimmune diseases. This reporter system offers sensitive and specific detection of antibody functions, improving drug development.

Keywords:

CCR8 HEK293-cAMP-biosensor-CCR8 engineered cell line functional evaluation monoclonal antibody

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Area of Science:

Immunology
Pharmacology
Biotechnology

Background:

Human chemokine receptor 8 (CCR8) is a key target for cancer and autoimmune disease immunotherapies.
Monoclonal antibodies targeting CCR8 demonstrate significant tumor growth inhibition and improved antitumor immunity.
Advancements in antibody evaluation methods are crucial for CCR8-targeted drug development.

Purpose of the Study:

To develop a novel engineered cell line for evaluating anti-CCR8 antibody functions.
To establish a sensitive and specific platform for assessing antibody specificity, biological activity, and effector functions.
To validate the utility of the reporter cell line with a newly developed CCR8 monoclonal antibody.

Main Methods:

Construction of a customized HEK293 cell line engineered with CCR8 and a cAMP-biosensor reporter.
Utilizing the reporter cell line to detect anti-CCR8 antibody specificity and biological activity.
Assessing antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP) using the reporter system.

Main Results:

Successful development of the HEK293-cAMP-biosensor-CCR8 reporter cell line.
Verification of biological activities of a new CCR8 monoclonal antibody (mAb 22H9) using the developed cell line.
Demonstration of high sensitivity and specificity of the reporter cell line for evaluating anti-CCR8 antibodies.

Conclusions:

The novel engineered cell line provides a robust platform for evaluating anti-CCR8 antibody functions.
This reporter system facilitates rapid kinetic detection and characterization of antibody efficacy.
The developed cell line supports the advancement of CCR8-targeted immunotherapies for cancer and autoimmune diseases.