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Published on: September 10, 2020
Integrated de novo transcriptome of Culex pipiens mosquito larvae as a resource for genetic control strategies
Valentina Mastrantonio1, Pietro Libro2, Jessica Di Martino2
1Department of Environmental Biology, Sapienza University of Rome, 00185, Rome, Italy.
Abstract:
We present a de novo transcriptome of the mosquito vector Culex pipiens, assembled by sequences of susceptible and insecticide resistant larvae. The high quality of the assembly was confirmed by TransRate and BUSCO. A mapping percentage until 94.8% was obtained by aligning contigs to Nr, SwissProt, and TrEMBL, with 27,281 sequences that simultaneously mapped on the three databases. A total of 14,966 ORFs were also functionally annotated by using the eggNOG database. Among them, we identified ORF sequences of the main gene families involved in insecticide resistance. Therefore, this resource stands as a valuable reference for further studies of differential gene expression as well as to identify genes of interest for genetic-based control tools.
