Related Experiment Video
Updated: Jul 9, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
Published on: July 10, 2014
Effect of the β-tricalcium phosphate particle on dental enamel associated with 10% carbamide peroxide bleaching
Marina-Paparotto Lopes1, Iana-Maria-Costa Gonçalves2, Julliana-Andrade da Silva2
1Undergraduate Student, Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas - UNICAMP, P.O. BOX 52, 13414-903, Piracicaba, SP, Brazil.
Background:
Since bleaching gels can cause adverse effects on tooth enamel, it is important to evaluate new remineralizing agents on the market and their effects.
Material And Methods:
Seventy-five bovine enamel/dentin blocks (4x4x3mm) were randomly divided into six groups (n=10): Negative Control (NC) with no bleaching treatment or brushing; 10 CP (Carbamide Peroxide) (no brushing - Whiteness Perfect FGM); CT12 + 10 CP (Colgate Total® 12); ES + 10 CP (Elmex® Sensitive); BPC + 10 CP (Bianco® ProClinical); CMP + 10 CP (Colgate® Máxima Proteção Anticáries). The color was evaluated by reflectance spectrophotometry (∆E*ab, ∆E00, and ∆WID) at times T1 (baseline), T2 (24 hours after brushing), and T3 (24 hours after bleaching). Knoop microhardness (KHN) analysis were performed at T3. The enamel surface was qualitatively analyzed by Scanning Electron Microscopy (SEM). The data were analyzed using generalized linear models through descriptive and exploratory analyses, and a significance level of 5% was considered.
Results:
Significant differences were observed when the bleached groups were compared to the NC group for ∆E*ab, ∆E00, and ∆WID at time T3 (p= <0.0001). However, the bleached groups presented no significant differences regarding studied times (p> 0.05). KHN did not differ significantly among the six groups (p=0.7585).
Conclusions:
Toothpastes with tricalcium phosphate (β-TCP) do not intervene with the efficacy of bleaching treatment with 10% carbamide peroxide. Although a slight mineral deposition on enamel surface can be observed on SEM images, KHN was not significantly altered, and the polishing of the samples were maintained. Key words:Dental Bleaching, Carbamide Peroxide, Hydrogen Peroxide, Dental Enamel, Tricalcium Phosphate.
More Related Videos
Related Concept Videos
Essential Minerals for Bone Health
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Biodeterioration

