Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Photoluminescence: Applications01:14

Photoluminescence: Applications

387
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
387
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

498
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
498
Reporter Genes02:11

Reporter Genes

11.3K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

4,4'- and 2,4'-Bis(olympicenyl)s: Antiferromagnetically Coupled Diradicals with Twisted Conformations.

Organic letters·2026
Same author

DFT Insights into <i>trans</i>-2-Silyl-4,5-dihydrofuran Formation via Au(I)-Catalyzed One-Pot Propargylation of Propargylsilanes and Aldehydes: Mechanism and Selectivities.

The Journal of organic chemistry·2026
Same author

Clinical fluorescent probes: mechanisms, advantages and inspirations.

Chemical science·2026
Same author

Spatiotemporal analysis of reactive oxygen species using specific activity-based sensing fluorescent imaging probes.

Nature reviews. Chemistry·2026
Same author

The Synthesis, Construction, and Applications of Benzophenone-Based Porous Polymers.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Artificial intelligence-assisted phenotyping of drug-resistant bacteria using a monosaccharide-based fluorescent sensor array.

Chemical science·2026
Same journal

Direct impure water electrolysis at industrial scale.

Chemical Society reviews·2026
Same journal

Catalytic valorization of polyolefins: from catalysts and processes to reactors.

Chemical Society reviews·2026
Same journal

Designing stable π-radicals.

Chemical Society reviews·2026
Same journal

Antibacterial drug discovery: challenges and preclinical promises from synthetic small molecules.

Chemical Society reviews·2026
Same journal

Selective carbon-carbon bond cleavage involving alkene moieties.

Chemical Society reviews·2026
Same journal

Circularly polarized luminescence: an easy path from molecules to supramolecular systems and beyond.

Chemical Society reviews·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2025

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
15:27

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

17.0K

Fluorescent small molecule donors.

Guang Chen1, Jing Yu1, Luling Wu2

  • 1The Youth Innovation Team of Shaanxi Universities, Shaanxi Key Laboratory of Chemical Additives for Industry, College of Chemistry and Chemical Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, China. chenandguang@163.com.

Chemical Society Reviews
|May 14, 2024
PubMed
Summary
This summary is machine-generated.

Fluorescent small molecule donors (FSMDs) offer solutions for controlling and monitoring small molecule release in disease treatment and drug development. This review details their design, triggering, and tracking for enhanced therapeutic efficacy and precision medicine.

More Related Videos

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

Published on: May 23, 2014

11.6K
Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
06:29

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Published on: July 30, 2020

3.1K

Related Experiment Videos

Last Updated: Jun 26, 2025

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
15:27

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

17.0K
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

Published on: May 23, 2014

11.6K
Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
06:29

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Published on: July 30, 2020

3.1K

Area of Science:

  • Chemical Biology
  • Pharmacology
  • Drug Development

Background:

  • Small molecule donors (SMDs) are crucial in signaling and disease treatment.
  • Challenges with SMDs include dosage control, targeted delivery, and real-time monitoring.
  • Fluorescent small molecule donors (FSMDs) address these challenges by enabling controllable release and non-invasive monitoring.

Purpose of the Study:

  • To review the design, triggering strategies, and tracking mechanisms of FSMDs.
  • To compile FSMDs for various small molecules (e.g., nitric oxide, carbon monoxide, hydrogen sulfide).
  • To discuss recent progress in molecular design, classification, generation, release, and fluorescence response.

Main Methods:

  • Systematic review of FSMDs for diverse small molecules.
  • Analysis of molecular design, structural classification, and generation mechanisms.
  • Examination of triggered release mechanisms (light, enzyme, ROS, biothiol, etc.) and fluorescence response.

Main Results:

  • Common structures for FSMD development and classification based on donor and fluorophore.
  • Established guidelines for optical properties and structure rearrangement in FSMDs.
  • Outlined applications in trackable release, monitoring, and in vivo treatment.

Conclusions:

  • FSMDs offer significant advantages for drug development, clinical diagnosis, and precision medicine.
  • Integration with therapeutic modalities enhances efficacy.
  • Further research is needed to address challenges for practical and clinical applications.