Related Experiment Video
Updated: Jun 26, 2025
08:25
Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
15.7K
Post-transcriptional (re)programming of B lymphocyte development: From bench to bedside?
Alia M Welsh1, Stefan A Muljo1
1Integrative Immunobiology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, United States.
Advances in Immunology
|May 19, 2024
Summary
Fetal liver hematopoietic stem cells (HSCs) can be reprogrammed into an adult-like state using RNA-binding proteins. This research explores their potential for in utero HSC transplantation and B-1a cell development.
Area of Science:
- Developmental biology
- Hematopoiesis
- Immunology
Background:
- Hematopoiesis undergoes significant changes during mammalian development, with definitive hematopoiesis marked by long-term hematopoietic stem cells (HSCs).
- Post-transcriptional differences exist between fetal liver (FL) and adult bone marrow (ABM) HSCs, with the transition mechanism remaining unclear.
- Fetal HSCs exhibit broader differentiation capacity, including innate-like lymphocytes, compared to adult HSCs.
Purpose of the Study:
- To investigate the post-transcriptional differences between FL and ABM HSCs.
- To explore the potential of RNA-binding proteins (RBPs) in reprogramming adult HSCs to a fetal-like state.
- To examine the role of RBPs, specifically LIN28B and IGF2BP3, in promoting B-1a cell development and their clinical application in HSC transplantation.
Main Methods:
- Comparative analysis of post-transcriptional profiles of FL and ABM HSCs.
- Investigating the function of specific RBPs (LIN28B, IGF2BP3) in HSC specification and differentiation.
- Evaluating the potential of synthetic co-expression of LIN28B and IGF2BP3 for HSC reprogramming and B-1a cell generation.
Main Results:
- Certain RBPs are crucial for HSC specification and can revert adult HSCs to a fetal-like state.
- LIN28B and IGF2BP3 are key RBPs expressed during fetal development that promote B-1a cell development.
- FL HSCs demonstrate a broader differentiation capacity, including the regeneration of conventional and unconventional lymphocytes.
Conclusions:
- Understanding RBPs offers a novel strategy for HSC reprogramming.
- LIN28B and IGF2BP3 hold promise for enhancing B-1a cell development.
- Synthetic co-expression of these RBPs may have future clinical applications in HSC transplantation and regenerative medicine.
Keywords:
B lymphocyte developmentFetal hematopoiesisHematopoietic stem cellsIn utero transplantationInnate-like lymphocytesMicroRNAPost-transcriptional regulationRNA-binding proteinMore Related Videos
Related Concept Videos
01:23Cells of the Adaptive Immune Response
980
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
980
01:24B Cell Activation and Differentiation
1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K
01:21Lineage Commitment
3.0K
Commitment is the process whereby stem cells:
3.0K
01:24Methods of Nuclear Reprogramming
1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
01:29Somatic to iPS Cell Reprogramming
2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K