Related Experiment Video
Updated: Jun 25, 2025
12:11
A Rhodopsin Transport Assay by High-Content Imaging Analysis
Published on: January 16, 2019
6.5K
Heliorhodopsin-mediated light-modulation of ABC transporter
Shin-Gyu Cho1,2, Ji-Hyun Kim1, Ji-Eun Lee1
1Department of Life Science, Sogang University, Seoul, South Korea.
Nature Communications
|May 21, 2024
Summary
This study reveals heliorhodopsins (HeRs) regulate multidrug resistance transporters. Light-activated HeR enhances drug transport, crucial for bacterial survival in drug-rich environments.
Area of Science:
- Microbiology
- Biochemistry
- Structural Biology
Background:
- Heliorhodopsins (HeRs) are photoreceptor proteins with hypothesized diverse functions.
- The specific roles of most HeRs remain largely uncharacterized.
- Understanding HeR functions is key to unlocking their potential applications.
Purpose of the Study:
- To investigate the light-modulation of heterodimeric multidrug resistance ATP-binding cassette transporters (OmrDE) by Omithinimicrobium cerasi HeR (OcHeR).
- To elucidate the molecular mechanisms underlying the interaction between OcHeR and OmrDE.
- To explore the functional implications of this interaction in the context of drug resistance.
Main Methods:
- Classification of genes flanking the HeR-encoding gene.
- Identification of conserved residues critical for protein-protein interactions.
- Thermodynamic analysis of the OcHeR-OmrDE binding interaction.
- Assessment of light-induced effects on OmrDE drug transport activity.
Main Results:
- The interaction between OcHeR and OmrDE exhibits positive cooperativity and sequential binding, as determined by thermodynamic parameters.
- Light activation of OcHeR leads to the upregulation of OmrDE-mediated drug transport.
- Conserved residues were identified as crucial for mediating the protein-protein interaction.
Conclusions:
- Heliorhodopsins function as regulatory rhodopsins controlling multidrug resistance.
- The light-induced regulation of OmrDE by OcHeR is vital for the survival of O. cerasi in drug-containing environments.
- These findings suggest potential applications for HeRs in optogenetic technologies.
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