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Updated: Jun 25, 2025

A Rhodopsin Transport Assay by High-Content Imaging Analysis
Published on: January 16, 2019
Heliorhodopsin-mediated light-modulation of ABC transporter
Shin-Gyu Cho1,2, Ji-Hyun Kim1, Ji-Eun Lee1
1Department of Life Science, Sogang University, Seoul, South Korea.
Abstract:
Heliorhodopsins (HeRs) have been hypothesized to have widespread functions. Recently, the functions for few HeRs have been revealed; however, the hypothetical functions remain largely unknown. Herein, we investigate light-modulation of heterodimeric multidrug resistance ATP-binding cassette transporters (OmrDE) mediated by Omithinimicrobium cerasi HeR. In this study, we classifiy genes flanking the HeR-encoding genes and identify highly conservative residues for protein-protein interactions. Our results reveal that the interaction between OcHeR and OmrDE shows positive cooperatively sequential binding through thermodynamic parameters. Moreover, light-induced OcHeR upregulates OmrDE drug transportation. Hence, the binding may be crucial to drug resistance in O. cerasi as it survives in a drug-containing habitat. Overall, we unveil a function of HeR as regulatory rhodopsin for multidrug resistance. Our findings suggest potential applications in optogenetic technology.
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