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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Related Experiment Video

Updated: Jun 25, 2025

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

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SLAM-Drop-seq reveals mRNA kinetic rates throughout the cell cycle.

Haiyue Liu1, Roberto Arsiè1, Daniel Schwabe2

  • 1Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.

Molecular Systems Biology
|May 22, 2024
PubMed
Summary

New SLAM-Drop-seq technology reveals mRNA kinetics. Most cycling genes require dynamic transcription and degradation for proper cell cycle regulation.

Keywords:
cell cyclemRNA kineticssingle cellstemporal regulationtranscription and degradation

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Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • RNA abundance is crucial for eukaryotic gene expression, regulated by production, processing, and degradation rates.
  • Understanding time-dependent kinetic rates in dynamic cellular processes remains challenging.
  • Existing methods lack the resolution to capture mRNA kinetics at the single-cell level during continuous biological processes.