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Estimating RNA Secondary Structure Folding Free Energy Changes with efn2

Jeffrey Zuber1, David H Mathews2,3

  • 1Department of Biochemistry & Biophysics and Center for RNA Biology, University of Rochester Medical Center, Rochester, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 23, 2024
PubMed
Summary

Estimating RNA folding free energy changes is crucial for analyzing RNA secondary structures. The efn2 program within RNAstructure provides these calculations, offering experimental uncertainty estimates and flexible usage options.

Keywords:
Equilibrium constantFolding free energyPseudoknotRNA secondary structureRNAstructureefn2

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