Related Experiment Video
Updated: Jun 25, 2025
07:14
Detection and Quantification of Calcitonin Gene-Related Peptide CGRP in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
Published on: June 16, 2023
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Calcitonin Gene-Related Peptide Level in Cystic Fibrosis Patients
Sabina Galiniak1, Marek Biesiadecki1, Iwona Rościszewska-Żukowska1
1Institute of Medical Sciences, Medical College, Rzeszów University, Warzywna 1a, 35-310 Rzeszów, Poland.
Life (Basel, Switzerland)
|May 25, 2024
Summary
Serum levels of alpha calcitonin gene-related peptide (αCGRP) are significantly elevated in cystic fibrosis (CF) patients compared to healthy individuals. This finding suggests a potential role for αCGRP in CF pathophysiology.
Area of Science:
- Respiratory Medicine
- Neuropeptide Research
- Genetic Disorders
Background:
- Calcitonin gene-related peptide (CGRP) is involved in respiratory tract physiology and pathophysiology.
- Limited data exist on the role of CGRP in cystic fibrosis (CF).
Purpose of the Study:
- To determine serum alpha CGRP (αCGRP) concentrations in patients with CF.
- To investigate the relationship between αCGRP levels and CFTR mutation types and disease severity.
Main Methods:
- Serum αCGRP levels were measured using enzyme-linked immunosorbent assay.
- 64 CF patients and 31 healthy controls were included.
- CF patients were analyzed based on CFTR mutation type and spirometry results.
Main Results:
- CF patients exhibited significantly higher serum αCGRP levels (62.51 ± 15.45 pg/mL) than controls (47.43 ± 8.06 pg/mL).
- Homozygotes for the ΔF508 mutation had higher αCGRP levels than heterozygotes.
- αCGRP levels were higher in patients with severe CF compared to mild CF.
Conclusions:
- Serum αCGRP is significantly elevated in CF patients.
- Increased αCGRP may play a role in CF pathophysiology.
- Further research is warranted to explore αCGRP's function in CF.
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