T84 Monolayer Cell Cultures Support Productive HBoV and HSV-1 Replication and Enable In Vitro Co-Infection Studies

Area of Science:

• Virology
• Cell Biology
• Molecular Biology

Background:

• Human bocaviruses (HBoV) are human pathogens, but their replication is challenging to study.
• Herpes Simplex Virus type 1 (HSV-1) is a common human herpesvirus.
• Previous research indicated HBoV-1 exclusively grew in complex air-liquid interface cultures.

Purpose of the Study:

• To investigate if T84 cells, a colorectal tumor-derived lung metastasis cell line, support HBoV-1 replication.
• To determine if T84 cells are permissive for HSV-1.
• To evaluate the potential of T84 cells as a model for HBoV-1 and HSV-1 co-infection studies.

Main Methods:

• Cultivating HBoV-1 and HSV-1 in T84 cell monolayers.
• Assessing HBoV-1 replication and viral DNA packaging in the presence of HSV-1.
• Utilizing T84 cells as a simplified cell culture system.

Main Results:

• T84 cells support HBoV-1 replication when cultured as monolayers.
• T84 cells are permissive for HSV-1 replication.
• HSV-1 positively influences HBoV-1 replication and the packaging of HBoV-1 progeny DNA into DNase-resistant particles.

Conclusions:

• T84 cell monolayers provide a viable and simpler model for HBoV-1 replication studies compared to air-liquid interface cultures.
• This model facilitates co-infection studies between HBoV-1 and HSV-1.
• HSV-1 appears to support HBoV-1 replication and virion formation, warranting further investigation and optimization for high-throughput studies.