Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Isotropic shrinkage of patterned vacancies enables three-dimensional nanoprecise metastructures for visible light applications.

Nature photonics·2026

Same author

Simultaneous brain-wide single-cell recording resolves spatiotemporal memory architecture.

bioRxiv : the preprint server for biology·2026

Same author

Subspace communication in the hippocampal-retrosplenial axis.

Nature·2026

Same author

Hierarchical Gating of Cortical Population Dynamics Drives Pain.

bioRxiv : the preprint server for biology·2026

Same author

A cautionary tale for AI and machine learning in psychiatry.

Translational psychiatry·2026

Same author

Time-frequency embedding with contrastive pre-training allows sub-second seizure detection.

bioRxiv : the preprint server for biology·2026

Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026

Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026

Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026

Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026

Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026

Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 25, 2025

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Pixel-wise programmability enables dynamic high-SNR cameras for high-speed microscopy.

Jie Zhang^1,2, Jonathan Newman^3,4, Zeguan Wang^4,5

¹Picower Institute for Learning and Memory, MIT, Cambridge, MA, USA. jzhang41@mit.edu.

Nature Communications

|May 27, 2024

Summary

This summary is machine-generated.

This study introduces a novel image sensor for high-speed fluorescence microscopy. It enhances signal-to-noise ratio, enabling detection of faint biological signals previously missed by standard cameras.

More Related Videos

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Related Experiment Videos

Last Updated: Jun 25, 2025

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Area of Science:

Biophotonics
Neuroscience
Microscopy Technology

Background:

High-speed wide-field fluorescence microscopy offers high spatiotemporal resolution for biological processes.
Conventional cameras struggle with low signal-to-noise ratio (SNR) at high frame rates, hindering detection of faint signals.

Purpose of the Study:

To develop an advanced image sensor with individually programmable pixel sampling speed and phase.
To improve signal quality and enable detection of weak biological events in high-speed imaging.

Main Methods:

Introduction of a novel image sensor with programmable pixel sampling.
Implementation in high-speed voltage imaging experiments.
Comparison with a low-noise scientific CMOS camera.

Main Results:

The new image sensor achieved a ~2-3 fold increase in output signal-to-noise ratio compared to a standard scientific CMOS camera.
Enabled detection of weak neuronal action potentials and subthreshold activities.
Demonstrated versatile sampling strategies for improved signal quality.

Conclusions:

The developed image sensor significantly enhances SNR in high-speed fluorescence microscopy.
It overcomes limitations of conventional cameras for detecting faint biological signals.
Offers flexible configurations for diverse experimental needs in biological imaging.