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Updated: Jun 25, 2025
Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
Published on: August 16, 2019
Embryonic development of the scorpion mud turtle (Kinosternon scorpioides) bred in captivity
Lianne Polliane Fernandes Araujo1, Diego Carvalho Viana2, Ligia Tchaika3
1Biotechnology (BIONORTE), São Luís, Brazil.
Background:
The developmental biology of Kinosternon scorpioides is described, based on the phenotype. This species is important for the flora because they are excellent seed disseminators. In addition, basic embryological information is not yet fully clarified, and this research provides unprecedented information on the chelonian embryology of the Amazonian fauna.
Aim:
The present study aims to identify the embryology of K. scorpioides in captivity during different periods.
Methods:
Females were monitored throughout the reproductive cycle, by video monitoring, to identify nests and the presence of newly laid eggs. At regular weekly intervals, embryo samples were collected fixed in a 4% paraformol solution and preserved in 70% alcohol. For the embryonic characterization, we used a stereomicroscope and the scanning electron microscopy method.
Results:
We describe 15 embryonic stages for a 15-week (105-day) incubation process. Only at 42 days (6th week) was the morphological characterization of a chelonian observed and at the 12th week (Stage XII), the phenotypic characterization of the species K. scorpioides.
Conclusion:
In view of the evidence, we found that these phases are similar to the other turtles, with structural variations in the appearance and disappearance of structures due to the specific characteristics of the species.
