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Updated: May 5, 2026

Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Amplification-free detection of Escherichia coli using an acidic deoxyribozyme-based paper device
Guangxiao Zhang1,2, Yunping Wu1,2, Wei Xue1,2
1School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China.
Abstract:
We reported a colorimetric paper-based device by integrating the modified acid RNA-cleaving DNAzymes (MaRCD-EC1) for highly sensitive (detection limit = 102 CFU mL-1), and rapid (within 30 min) detection of E. coli without amplification. This device exhibited a clinical sensitivity of 100% and a specificity of 100% in identifying E. coli-associated urinary tract infections (UTIs) using the clinical urine samples.
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