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Updated: Jun 24, 2025
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay
Published on: September 2, 2014
Protocol to detect telomerase activity in adult mouse hippocampal neural progenitor cells using the telomeric repeat
Diji Kuriakose1, Zhi-Cheng Xiao2
1Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC 3800, Australia.
Abstract:
Changes in telomerase activity and telomere length contribute to aging-related decline. Investigating telomerase in aging models provides insights into related pathologies. Here, we present a protocol to detect telomerase activity in adult mouse hippocampal neural progenitor cells using the telomeric repeat amplification protocol assay. We describe steps for isolating and expanding aged mouse hippocampal neural progenitor cells (NPCs) and assessing telomerase using a non-radioactive technique. The protocol emphasizes the significance of understanding telomerase activity in NPCs for neurogenesis and age-related diseases.
