Whole blood multiplex measurements using electrochemical aptamer-based biosensors

Area of Science:

• Electrochemistry
• Biosensor Technology
• Molecular Diagnostics

Background:

• Multiplex molecular measurements using electrochemical biosensors often require multiple electrodes, posing challenges for specific patient populations like neonates and hemophiliacs.
• Existing methods are not ideal for continuous, real-time analysis in complex biological samples such as whole blood.

Purpose of the Study:

• To develop a single-electrode electrochemical aptamer-based biosensor capable of true multiplex detection.
• To enable continuous and real-time measurement of two distinct molecules simultaneously in undiluted whole blood.

Main Methods:

• Incorporation of the oxazine ATTO 700 dye into electrochemical aptamer-based sensor design.
• Testing the sensor's performance for simultaneous detection of two target molecules in undiluted whole blood samples.

Main Results:

• Demonstrated the feasibility of achieving "true" multiplex detection using a single electrode.
• Successfully performed continuous and real-time measurements of two different molecules in undiluted whole blood.

Conclusions:

• The developed oxazine ATTO 700-based electrochemical aptamer biosensor offers a simplified approach for multiplex analysis.
• This single-electrode system provides a promising platform for real-time diagnostics in challenging biological matrices, overcoming limitations of multi-electrode systems.