Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibitory effect of the water-soluble retinoid X receptor partial agonist CAt-PMN on allergic conjunctivitis-like responses in mice.

Experimental eye research·2026
Same author

Effects of Inhalation of Hydrogen Gas on Allergic Conjunctivitis Model Mice.

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics·2026
Same author

Influences of age and sex on allergic symptoms in food allergy model mice.

Immunology letters·2026
Same author

A mouse model of food allergy permitting skin and nasal symptoms.

Advances in medical sciences·2023
Same author

A mouse model of allergic conjunctivitis permitting tear eosinophil quantification.

Journal of pharmacological and toxicological methods·2022
Same author

Involvement of glucocorticoid receptor activation on anti-inflammatory effect induced by peroxisome proliferator-activated receptor γ agonist in mice.

International immunopharmacology·2014

Related Experiment Video

Updated: Jun 24, 2025

Establishment of A Mouse Model of Aqueous Deficiency Dry Eye
05:21

Establishment of A Mouse Model of Aqueous Deficiency Dry Eye

Published on: November 1, 2024

560

Evaluation methods using tear volume in a conjunctivitis mice model.

Haruki Kai1, Noriaki Haraoka1, Yukio Sugimoto1

  • 1Department of Inflammatory Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.

Journal of Pharmacological and Toxicological Methods
|June 3, 2024
PubMed
Summary

This study developed a quantitative method to evaluate allergic conjunctivitis in mice by measuring tear volume. This model effectively assesses drug efficacy for allergic eye conditions, identifying histamine

Keywords:
CarrageenanCompound 48/80ConjunctivitisHistamineMethodsMiceOvalbuminTear volume

More Related Videos

Induction of Ocular Surface Inflammation and Collection of Involved Tissues
06:38

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Published on: August 4, 2022

2.3K
A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies
08:04

A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies

Published on: January 24, 2020

12.4K

Related Experiment Videos

Last Updated: Jun 24, 2025

Establishment of A Mouse Model of Aqueous Deficiency Dry Eye
05:21

Establishment of A Mouse Model of Aqueous Deficiency Dry Eye

Published on: November 1, 2024

560
Induction of Ocular Surface Inflammation and Collection of Involved Tissues
06:38

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Published on: August 4, 2022

2.3K
A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies
08:04

A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies

Published on: January 24, 2020

12.4K

Area of Science:

  • Ophthalmology
  • Immunology
  • Pharmacology

Background:

  • Allergic conjunctival disease is an immune-mediated inflammatory condition.
  • Developing quantitative evaluation methods for animal models is crucial for clinically useful drug development.
  • Current methods for assessing allergic conjunctivitis in animal models lack quantitative precision.

Purpose of the Study:

  • To establish a quantitative evaluation method for allergic conjunctivitis in a mouse model.
  • To assess the efficacy of drugs in an antigen-induced allergic conjunctivitis mouse model.
  • To investigate the role of histamine in allergic conjunctivitis-induced tear volume.

Main Methods:

  • Allergic conjunctivitis was induced in mice via systemic and local ovalbumin (OVA) sensitization.
  • Tear volume was measured as a primary endpoint to evaluate conjunctivitis severity.
  • The effects of histamine, compound 48/80, carrageenan, levocabastine, and betamethasone on tear volume were assessed.

Main Results:

  • Antigen (OVA) challenge induced increased tear volume in the sensitized mouse model.
  • Histamine, compound 48/80, and carrageenan directly increased tear volume upon ocular instillation.
  • Levocabastine inhibited tear volume in OVA-, histamine-, and compound 48/80-induced models; betamethasone inhibited carrageenan-induced tear volume.

Conclusions:

  • Tear volume measurement in this mouse model provides a quantitative assessment of allergic conjunctivitis.
  • Histamine appears to play a role in increased tear volume during allergic conjunctivitis.
  • This model is a valuable tool for evaluating the efficacy of drugs for allergic conjunctivitis.