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Induction of Ocular Surface Inflammation and Collection of Involved Tissues

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Published on: August 4, 2022

A mouse model of allergic conjunctivitis permitting tear eosinophil quantification.

Atsushi Ogura¹, Yukio Sugimoto¹

¹Department of Inflammatory Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.

Journal of Pharmacological and Toxicological Methods

|September 21, 2022

Summary

A new ovalbumin-induced mouse model effectively mimics allergic conjunctivitis symptoms. This model, using eosinophil counts in tears, offers a quantitative, objective, and noninvasive method for evaluating allergic conjunctivitis severity.

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Area of Science:

Ophthalmology
Immunology
Allergy Research

Background:

Allergic conjunctivitis is an antigen-induced immune-mediated inflammatory eye disease.
Key symptoms include ocular itching, redness (hyperemia), and swelling (edema).
Developing reliable animal models and objective evaluation methods is crucial for understanding and treating allergic conjunctivitis.

Purpose of the Study:

To develop an ovalbumin (OVA)-induced mouse model of allergic conjunctivitis.
To establish a quantitative and objective method for evaluating allergic conjunctivitis symptoms.

Main Methods:

ICR mice were sensitized with ovalbumin (OVA) via intraperitoneal injection and ocular instillation.
Mice received daily topical treatments of betamethasone, levocabastine, or PBS (control) for 14 days.

Keywords:

Allergic conjunctivitis Drug development Eosinophil Methods Mice Tear

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Symptoms evaluated included scratching, hyperemia, edema, tear eosinophil infiltration, and immunoglobulin E (IgE) levels.

Main Results:

The OVA-induced model exhibited characteristic allergic conjunctivitis symptoms like eye scratching, hyperemia, and edema.
Elevated antigen-specific IgE levels were observed in tears and serum.
Topical treatments with betamethasone and levocabastine significantly inhibited these allergic symptoms.

Conclusions:

The developed OVA-induced mouse model accurately replicates clinical allergic conjunctivitis.
Measuring eosinophils in tears using phenol red threads provides a quantitative, objective, and noninvasive evaluation method.
This methodology is valuable for both experimental research and clinical assessment of allergic conjunctivitis severity.