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Related Experiment Video

Updated: Jun 24, 2025

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform
13:24

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

Published on: September 10, 2009

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A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy.

Federico Nebuloni1,2, Quyen B Do3,4,5, Peter R Cook2

  • 1Osney Thermofluids Institute, Department of Engineering Science, University of Oxford, Osney Mead, Oxford OX2 0ES, UK. edmond.walsh@eng.ox.ac.uk.

Lab on a Chip
|June 6, 2024
PubMed
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Researchers developed a novel microfluidic system for building and studying human neuronal circuits in vitro. This accessible technology enables the creation of functional cortico-striatal connections and axotomy models for drug screening.

Area of Science:

  • Neuroscience
  • Biotechnology
  • Stem Cell Biology

Background:

  • Establishing functional neuronal circuits in vitro is crucial for understanding brain function and disease.
  • Existing in vitro models often lack the complexity and accessibility needed for widespread biomedical research.

Purpose of the Study:

  • To develop an accessible and versatile in vitro microfluidic system for constructing and analyzing human neuronal circuits.
  • To model unidirectional cortico-striatal connectivity and create a platform for studying axon regeneration and drug screening.

Main Methods:

  • Utilized an open microfluidic system with fluid walls (FC40) to create dumbbell-shaped neuronal circuits in Petri dishes.
  • Cultured post-mitotic neurons derived from human induced pluripotent stem cells (iPSCs) within the microfluidic chambers.

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Last Updated: Jun 24, 2025

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  • Developed a non-contact axotomy assay using a media jet to sever axons within microfluidic conduits.

    • Main Results:

    • Successfully constructed dumbbell-shaped neuronal circuits mimicking unidirectional cortico-striatal connectivity within minutes.
    • Demonstrated that severed cortical axons in conduits can regenerate, promoted by brain-derived neurotrophic factor and striatal neurons.
    • The system allows for easy addition of conduits and chambers, enabling the creation of more complex neuronal networks.

    Conclusions:

    • The developed microfluidic platform provides an accessible and efficient method for building and studying human neuronal circuits in vitro.
    • This system facilitates the investigation of neuronal connectivity, axon regeneration, and offers a valuable tool for drug discovery and neurodegenerative disease research.