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Updated: Jul 17, 2026
High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
Published on: May 19, 2011
Protocol for forward genetic screening to identify novel factors involved in a biological process in Caenorhabditis
Keita Yoshida1, Yuji Suehiro1, Shohei Mitani2
1Department of Physiology, Tokyo Women's Medical University School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Abstract:
Forward genetic screens have been a powerful tool for discovering genes involved in various biological processes in Caenorhabditis elegans. Here, we present a protocol for forward genetic screening to identify novel factors involved in a biological process in C. elegans. We describe steps for mutagenesis, screening, and backcrossing. To save time and effort, we also detail procedures for utilizing whole-genome sequencing to exclude mutants of previously characterized genes from crosses for mapping mutations. For complete details on the use and execution of this protocol, please refer to Yoshida et al.1.
