Multifunctional Metal-Organic Frameworks Driven Three-Dimensional Folded Paper-Based Microfluidic Analysis Device for...

Area of Science:

• Analytical Chemistry
• Materials Science
• Environmental Science

Background:

• Chlorpyrifos (CPF) residues in food present significant risks to human health and ecosystems.
• Accurate and rapid detection methods are crucial for monitoring food safety and environmental contamination.

Purpose of the Study:

• To develop a novel three-dimensional folded paper-based microfluidic analysis device (3D-μPAD) for the rapid quantitative detection of chlorpyrifos (CPF).
• To utilize multifunctional metal-organic frameworks and upconversion nanomaterials for a dual-mode fluorescence-colorimetric readout.

Main Methods:

• Fabrication of a 3D-μPAD by integrating upconversion nanomaterials with a bimetal organic framework exhibiting peroxidase activity, forming a fluorescence-quenched nanoprobe.
• Loading the nanoprobe onto the paper-based detection zone and applying a color-developing solution for dual-mode detection.
• Quantitative analysis of CPF by measuring fluorescence recovery and color change (colorless to blue).

Main Results:

• The 3D-μPAD demonstrated a good linear relationship between CPF concentration and detection signal (fluorescence intensity and color change).
• Achieved low limits of detection: 0.028 ng/mL (fluorescence) and 0.043 ng/mL (colorimetric).
• The device showed comparable results to high-performance liquid chromatography (HPLC) when applied to real food samples.

Conclusions:

• The developed 3D-μPAD offers a sensitive, rapid, and reliable platform for on-site detection of chlorpyrifos residues in food.
• This technology holds significant potential for improving food safety monitoring and environmental surveillance.
• The dual-mode readout enhances the robustness and accessibility of the detection method.