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Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
Published on: February 23, 2018
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Toto-Cell: A new software to analyze cellular events during video-microscopy
Léa-Isabelle Renaud1, Christophe Renaud2, Samuel Delepoulle2
1Laboratoire de Gynéco-Oncologie Moléculaire, Université du Québec Trois-Rivières, Trois Rivières, Québec, Canada.
Plos One
|June 21, 2024
Summary
Toto-Cell is a new software that simplifies video-microscopy data analysis for cell behavior studies. It quantifies cell parameters, improving accuracy over manual methods.
Area of Science:
- Cell biology
- Bioimaging
- Software development
Background:
- Video-microscopy is crucial for single-cell behavior analysis.
- Manual tracking of real-time cellular events is time-consuming and prone to error.
- AI-driven analysis is emerging but manual intervention is still required for some events.
Purpose of the Study:
- To develop a user-friendly software, Toto-Cell, for analyzing video-microscopy data.
- To enable quantification of previously difficult-to-measure cellular parameters.
- To facilitate the analysis of cellular events induced by various treatments.
Main Methods:
- Developed Toto-Cell software for video-microscopy data analysis.
- Utilized HEC-1-A endometrial cell line for experiments.
- Treated cells with cisplatin, doxorubicin, and AZD5363 (Akt inhibitor).
- Tracked parameters: mitosis (normal/abnormal), cell death/fusion ratios, and cell cycle length.
Main Results:
- Toto-Cell successfully quantified various cellular parameters.
- Demonstrated significant heterogeneity in cellular responses within single treatments.
- Highlighted previously challenging parameters like mitosis and cell death ratios.
Conclusions:
- Toto-Cell is an effective tool for quantitative and qualitative analysis of video-microscopy data.
- The software enhances accuracy compared to manual calculations.
- It aids researchers in understanding complex cellular dynamics and treatment effects.
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