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Related Concept Videos

CRISPR01:59

CRISPR

50.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Jun 23, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Genome editing using CRISPR, CAST, and Fanzor systems.

Beomjong Song1, Sangsu Bae2

  • 1Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 33151, Republic of Korea.

Molecules and Cells
|June 23, 2024
PubMed
Summary

Genetic engineering advances, including CRISPR-Cas systems, enable precise gene editing in animal models for research and therapeutics. New systems like CASTs and OMEGA expand genome editing capabilities.

Keywords:
Clustered regularly interspaced short palindromic repeats-associated proteinClustered regularly interspaced short palindromic repeats-associated transposonsFanzorGenome editingIscBTnpB

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Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Genetic engineering is crucial for basic science and developing therapeutic animal models.
  • The CRISPR-Cas system revolutionized genome editing with its precise, guide RNA-dependent targeting.
  • Novel CRISPR-associated transposons (CASTs) and OMEGA RNA systems offer new genome editing tools.

Purpose of the Study:

  • To review various CRISPR-Cas systems and their applications.
  • To highlight advancements in genome editing technologies.
  • To discuss the utility of emerging systems like CASTs and OMEGA RNA.

Main Methods:

  • Review of scientific literature on CRISPR-Cas systems.
  • Analysis of diverse CRISPR-Cas system features.
  • Examination of genome editing applications in animal models.

Main Results:

  • CRISPR-Cas systems provide precise gene editing capabilities.
  • Emerging systems (CASTs, OMEGA RNA, Fanzor) expand genome editing toolkits.
  • These technologies are applicable across various animal models for research and therapy.

Conclusions:

  • CRISPR-Cas and related systems are powerful tools for genetic engineering.
  • Ongoing research into novel systems promises further advancements in genome editing.
  • Applications in animal models are vital for advancing basic science and therapeutic development.