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Related Concept Videos

Diabetic Nephropathy01:28

Diabetic Nephropathy

Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration occur due to afferent arteriolar...

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Related Experiment Video

Updated: Jun 22, 2026

Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease
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Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease

Published on: May 10, 2024

551

Quantifying Protein Acetylation in Diabetic Nephropathy from Formalin-Fixed Paraffin-Embedded Tissue.

Stefanie K Schwab1, Peter S Harris1, Cole Michel1

  • 1Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.

Proteomics. Clinical Applications
|June 26, 2024
PubMed
Summary

Diabetic kidney disease alters protein acetylation, impacting key metabolic pathways. This study developed a new method to quantify these changes in kidney tissue, aiding biomarker discovery.

Keywords:
acetylationdiabetesformalin‐fixed paraffin‐embeddedkidney diseaseproteomics

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Area of Science:

  • Biochemistry
  • Proteomics
  • Nephrology

Background:

  • Diabetic kidney disease (DKD) is a major complication of diabetes, leading to kidney failure.
  • Altered protein modifications, like lysine acetylation, may drive DKD pathogenesis.
  • Lysine acetylation is linked to cellular metabolism and potentially impaired in diabetes.

Purpose of the Study:

  • To develop and apply a novel quantitative acetylomics approach for formalin-fixed paraffin-embedded (FFPE) kidney tissue.
  • To identify changes in protein acetylation in patients with DKD compared to non-diabetic controls.
  • To explore the functional impact of altered acetylation on metabolic pathways in DKD.

Main Methods:

  • Adapted a novel extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique.
  • Quantified lysine acetylation sites in FFPE kidney biopsies from DKD patients (n=5) and controls (n=7).
  • Analyzed changes in protein and peptide acetylation levels and associated pathways.

Main Results:

  • Identified 840 total proteins, with 225 significantly altered in DKD.
  • Quantified 289 acetylated peptides, with 69 showing significant changes in DKD.
  • Observed significant impacts on metabolic pathways including mitochondrial function, oxidative phosphorylation, and sirtuin signaling.

Conclusions:

  • Developed a quantitative acetylomics platform for FFPE kidney biopsies.
  • Demonstrated differential protein acetylation in DKD, affecting key metabolic processes.
  • This platform facilitates protein biomarker discovery for DKD in kidney transplant patients.