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3D Multispheroid Assembly Strategies towards Tissue Engineering and Disease Modeling
Tong Zhu1, Yan Hu1, Haitao Cui1
1Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, China.
Advanced Healthcare Materials
|June 26, 2024
Summary
Multispheroid assembly uses cell spheroids as building blocks to create advanced 3D tissue models. This technique enhances disease modeling and tissue engineering by mimicking natural tissue complexity.
Area of Science:
- Biotechnology
- Tissue Engineering
- 3D Cell Culture
Background:
- Cell spheroids and organoids are valuable 3D models for studying cell-cell and cell-extracellular matrix (ECM) interactions.
- Complex 3D cell models with multiple cell types are crucial for understanding tissue pathophysiology and developing in vitro disease models.
Purpose of the Study:
- To provide a comprehensive overview of recent advances in multispheroid assembly techniques.
- To introduce different mechanisms of multispheroid assembly and summarize their processing steps, advantages, and limitations.
Main Methods:
- Overview of automated directed assembly, noncontact remote assembly, and programmed self-assembly strategies.
- Summary of methodologies for constructing complex 3D tissue models using cell spheroids as living building blocks.
Main Results:
- Detailed review of multispheroid assembly techniques for creating spatially organized 3D tissue models.
- Exploration of applications in disease modeling, drug screening, tissue engineering, and organogenesis.
Conclusions:
- Multispheroid assembly offers a promising approach for generating advanced 3D cell models that better recapitulate native tissue complexity.
- Highlights persistent challenges and future research directions to further advance the field of 3D tissue model development.
