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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
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Self-designed portable dual-mode fluorescence device with custom python-based analysis software for rapid detection
Chengquan Wang1, Chengdong Gu1, Xin Zhao1
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Food Chemistry
|June 26, 2024
Summary
A novel aptasensor detects ochratoxin A (OTA) and aflatoxin B1 (AFB1) in corn using MoS2-modified quantum dots and a portable spectrometer. This IoT-enabled device offers convenient and reliable fungal toxin detection for enhanced food safety.
Area of Science:
- Analytical Chemistry
- Nanoscience
- Food Science
Background:
- Ochratoxin A (OTA) and aflatoxin B1 (AFB1) are common mycotoxins in food, posing significant health risks.
- Accurate and rapid detection methods for these toxins are crucial for food safety.
- Existing detection methods can be time-consuming, expensive, or require specialized laboratory equipment.
Purpose of the Study:
- To develop an innovative aptasensor for the simultaneous detection of OTA and AFB1.
- To integrate the aptasensor with a portable spectrometer and IoT capabilities for on-site analysis.
- To provide a rapid, convenient, and reliable method for fungal toxin detection in food matrices.
Main Methods:
- Fabrication of a MoS2-modified aptasensor using bicolor quantum dots (carbon dots and CdZnTe quantum dots) as nano-donors.
- Labeling of OTA and AFB1 aptamers with respective nano-donors and attachment to MoS2 receptors.
- Utilizing fluorescence resonance energy transfer (FRET) for target detection, where target binding disrupts FRET and causes fluorescence recovery.
- Development of a portable dual-mode fluorescence detection system with custom Python software and cloud connectivity (IoT).
Main Results:
- The developed aptasensor successfully achieved simultaneous detection of OTA and AFB1.
- The FRET mechanism enabled sensitive detection based on fluorescence recovery upon target binding.
- The portable spectrometer and IoT integration facilitated rapid, convenient, and remote data transmission.
- The system demonstrated potential for on-site food safety monitoring.
Conclusions:
- The MoS2-modified bicolor quantum dot aptasensor provides a sensitive and selective platform for simultaneous OTA and AFB1 detection.
- The portable, IoT-enabled detection system offers a promising solution for rapid and reliable food safety analysis.
- This technology can contribute to improved monitoring and control of mycotoxins in agricultural products like corn.
