Related Experiment Video
Updated: Jun 22, 2025
07:24
Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
Published on: June 29, 2017
9.0K
EPS Production by Lacticaseibacillus casei Using Glycerol, Glucose, and Molasses as Carbon Sources
Guilherme Deomedesse Minari1, Rodolfo Debone Piazza2, Daiane Cristina Sass1
1Institute of Biosciences, São Paulo State University (Unesp), Rio Claro 13506-900, Brazil.
Microorganisms
|June 27, 2024
Summary
Lacticaseibacillus casei efficiently produces exopolysaccharides (EPS) using agro-industrial byproducts like molasses and glycerol. This sustainable approach utilizes inexpensive carbon sources for valuable polysaccharide extraction.
Area of Science:
- Microbiology
- Biochemistry
- Biotechnology
Background:
- Exopolysaccharides (EPS) are crucial biomolecules with diverse applications.
- Traditional EPS production often relies on expensive carbon sources.
- Exploring sustainable alternatives for EPS synthesis is vital for industrial viability.
Purpose of the Study:
- To investigate the potential of Lacticaseibacillus casei to produce exopolysaccharides (EPS) using alternative, cost-effective carbon sources.
- To characterize the EPS produced from different carbon sources.
- To evaluate the feasibility of utilizing agro-industrial residues for EPS production.
Main Methods:
- Screening of 22 Lactobacillus strains for optimal EPS production at 37°C.
- Selection of Lacticaseibacillus casei strain Ke8 for further studies.
- Characterization of EPS using High-Performance Liquid Chromatography (HPLC), Gas Chromatography-Mass Spectrometry (GC-MS), Fourier-Transform Infrared Spectroscopy (FTIR), and Proton Nuclear Magnetic Resonance (¹H-NMR).
Main Results:
- Lacticaseibacillus casei strain Ke8 demonstrated efficient EPS production using glycerol and sugarcane molasses.
- EPS from glycerol/glucose comprised glucose and mannose (1730 g/mol), with 39.4% carbohydrates and 18% proteins.
- EPS from molasses contained glucose, galactose, and arabinose (1182 g/mol), with 52.9% carbohydrates and 11.69% proteins.
- FTIR and NMR analyses confirmed the polysaccharide nature of the produced molecules.
Conclusions:
- Lacticaseibacillus casei can effectively utilize alternative carbon sources like glycerol and molasses for EPS production.
- Agro-industrial residues offer a sustainable and economical substrate for EPS synthesis.
- This study highlights a novel application of Lacticaseibacillus casei in producing EPS from renewable resources, addressing a gap in current research.
