Enhancing Dengue Virus Production and Immunogenicity with Celcradle™ Bioreactor: A Comparative Study with Traditional...

Area of Science:

• Virology
• Biotechnology
• Vaccinology

Background:

• Dengue virus is a major global health concern, causing significant morbidity and mortality worldwide.
• Existing dengue vaccine development faces challenges in efficacy and large-scale production.
• There is a critical need for improved methods for dengue virus cultivation and vaccine production.

Purpose of the Study:

• To evaluate the Celcradle™ bioreactor for high-density Vero cell culture and dengue virus production.
• To assess the immunogenicity of inactivated dengue virus produced using the Celcradle™ system.
• To compare the efficiency and effectiveness of Celcradle™-based production with traditional methods.

Main Methods:

• Utilized Celcradle™, a tidal bioreactor, for high-density Vero cell culture.
• Optimized pH (7.0-7.4) and glucose (1.5-3.5 g/L) for cell and virus proliferation.
• Produced inactivated DENV4 and assessed its immunogenicity in mice.

Main Results:

• Achieved a peak Vero cell count of approximately 2.46 × 10^9, a nearly tenfold increase.
• Significantly reduced time for cell yield and virus production compared to Petri dishes.
• Celcradle™-produced virus showed enhanced immunogenicity and elevated antibody titers in mice.

Conclusions:

• The Celcradle™ bioreactor is a promising platform for large-scale inactivated dengue virus vaccine production.
• This system offers a more efficient and effective method for generating immunogenic dengue virus.
• Celcradle™ technology could accelerate the development of effective dengue vaccination strategies globally.