Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

134
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
134
Positron Emission Tomography01:29

Positron Emission Tomography

4.2K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
4.2K
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

105
Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
105
Radiological Investigation III: Pulmonary Angiogram and PET Scan01:13

Radiological Investigation III: Pulmonary Angiogram and PET Scan

91
Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...
91
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

321
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
321

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fully Automated Azeotropic Drying-Free Synthesis of [<sup>18</sup>F]SynVesT-1 via Copper-Mediated Fluorination Using the Trasis All-in-One Module.

Molecules (Basel, Switzerland)·2026
Same author

Integration of functional immunomonitoring assays with PET/CT scans in TB patients identifies on-treatment biomarkers.

bioRxiv : the preprint server for biology·2026
Same author

B7-H4-targeted radiotheranostics enable precise imaging and potent therapy across solid tumor models.

Science advances·2026
Same author

Regolith as a Refuge: Differential Survival of Bacteriophage Qβ in Mars Analog Environments.

Astrobiology·2026
Same author

Synthesis of 4-Aminoindole-Containing Cephalosporin Prodrugs and Their Biological Activity against <i>Mycobacterium tuberculosis</i>.

ACS infectious diseases·2026
Same author

Development of novel GPC2-directed radiotheranostics and CAR T cell therapy for neuroblastoma.

Molecular therapy : the journal of the American Society of Gene Therapy·2026

Related Experiment Video

Updated: Jun 22, 2025

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates
10:04

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Published on: September 5, 2017

18.8K

Distributable, metabolic PET reporting of tuberculosis.

R M Naseer Khan1,2, Yong-Mo Ahn3, Gwendolyn A Marriner3

  • 1Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Oxford, UK.

Nature Communications
|June 27, 2024
PubMed
Summary

A novel positron-emitting tracer, 2-[18F]fluoro-2-deoxytrehalose ([18F]FDT), specifically images Mycobacterium tuberculosis (Mtb) by targeting its unique trehalose metabolism. This breakthrough offers a new tool for tuberculosis diagnosis and treatment monitoring.

More Related Videos

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
10:04

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

12.0K
Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT
07:07

Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT

Published on: May 2, 2017

14.0K

Related Experiment Videos

Last Updated: Jun 22, 2025

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates
10:04

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Published on: September 5, 2017

18.8K
Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
10:04

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

12.0K
Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT
07:07

Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT

Published on: May 2, 2017

14.0K

Area of Science:

  • Biomedical Imaging
  • Radiochemistry
  • Infectious Diseases

Background:

  • Tuberculosis (TB) presents a significant global health challenge, marked by lengthy treatment and difficult disease monitoring.
  • Current diagnostic methods, primarily sputum culture, have limitations in detecting TB and assessing treatment efficacy.
  • Existing imaging agents like [18F]FDG lack specificity for Mycobacterium tuberculosis (Mtb), hindering direct correlation with pathogen viability.

Purpose of the Study:

  • To develop and validate a novel positron-emitting tracer for specific in vivo imaging of Mycobacterium tuberculosis (Mtb).
  • To assess the utility of the tracer in diverse disease models for monitoring TB lesions and treatment response.
  • To establish a practical and scalable method for the synthesis of the tracer from a widely available precursor.

Main Methods:

  • Synthesis of 2-[18F]fluoro-2-deoxytrehalose ([18F]FDT), a mimic of Mtb's disaccharide trehalose.
  • In vivo imaging studies using [18F]FDT in various TB disease models, including non-human primates.
  • Evaluation of a direct, enzyme-catalyzed radiochemical synthesis of [18F]FDT from [18F]FDG.

Main Results:

  • [18F]FDT demonstrated mechanism-based reporter activity, specifically imaging Mtb via Mtb-mediated trehalose processing.
  • Successful imaging of TB-associated lesions and monitoring of treatment effects were achieved in diverse preclinical models.
  • A pyrogen-free, direct enzyme-catalyzed synthesis enabled efficient production of [18F]FDT from [18F]FDG.

Conclusions:

  • [18F]FDT is a bacterium-selective PET tracer that specifically targets Mtb, offering a significant advancement over current imaging methods.
  • The developed synthesis method allows for the production of clinical-grade [18F]FDT from the widely available [18F]FDG.
  • This technology has the potential to democratize access to TB-specific PET imaging globally, aiding diagnosis and treatment management.