Characterization of temperature-sensitive Schizosaccharomyces pombe mutants in the septation initiation network Spg1...

The Schizosaccharomyces pombe GTPase, Spg1 , activates the septation initiation network (SIN) protein kinase cascade to trigger septation. In the absence of functional Spg1 , cells fail cytokinesis and become multinucleate. In this study, we characterize a set of temperature-sensitive spg1 alleles isolated in the 1990s. We identify the mutations within each new and previously characterized allele, characterize the extent of relative growth defects, and assess their interaction with other SIN alleles.