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Updated: Jun 22, 2025
An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
Extraction of Proteins from Marine Microbial Communities Sampled by Lab Filters
Timothy J Williams1,2, Ricardo Cavicchioli3
1School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, Australia. tim.williams@uts.edu.au.
Abstract:
A method for the recovery of whole-cell protein extracts from biomass on membrane filters is provided here. The protein extraction method is ideal for biomass captured by filtration of large water volumes, including seawater from marine environments. The protein extraction method includes both chemical disruption and physical disruption to lyse cells and release protein for subsequent metaproteomic analysis.
