Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.7K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

343
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
343

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring Chiral Exceptional Lines in the Visible Regime.

Physical review letters·2026
Same author

A Cost-Effective, Chip-Based Platform for Patterned Single-Cell Culture.

ACS applied bio materials·2026
Same author

Correction: Objective interpretation of intrapartum cardiotocography images using attention-guided convolutional neural networks.

Frontiers in pediatrics·2026
Same author

Mechanistic Insights into the Transient Reactions of Environmentally Persistent Free Radicals on Common Microplastics: An Important Role of Air Humidity.

Environmental science & technology·2026
Same author

The rising global burden of gout attributable to kidney dysfunction: a 30-year trend analysis and projections to 2036.

Clinical rheumatology·2026
Same author

Reshaping tumor immunity by targeting SUMOylation: A novel immunotherapeutic strategy.

Biochimica et biophysica acta. Reviews on cancer·2026
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2025

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

13.5K

Three-dimensional rainbow refractometry.

Zhiwen Deng, Yingchun Wu, Xinhao Wang

    Optics Letters
    |July 1, 2024
    PubMed
    Summary
    This summary is machine-generated.

    We introduce three-dimensional rainbow refractometry (TDRR), a novel technique for precisely measuring droplet properties. This method accurately determines droplet size, refractive index, and 3D position using rainbow light scattering.

    More Related Videos

    Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb
    06:50

    Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

    Published on: December 2, 2017

    9.2K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    6.9K

    Related Experiment Videos

    Last Updated: Jun 22, 2025

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    13.5K
    Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb
    06:50

    Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

    Published on: December 2, 2017

    9.2K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    6.9K

    Area of Science:

    • Optical physics
    • Fluid dynamics
    • Metrology

    Background:

    • Rainbow refractometry is a valuable technique for non-invasive droplet analysis.
    • Existing methods often lack the capability for simultaneous 3D positioning.

    Purpose of the Study:

    • To develop and validate a novel three-dimensional rainbow refractometry (TDRR) technique.
    • To enable accurate measurement of droplet size, refractive index, and axial position.

    Main Methods:

    • Integration of a cylindrical lens into the signal collection system.
    • Development of a TDRR model based on ray transfer matrix.
    • Calibration of rainbow scattering angle to system parameters.
    • Implementation of a new rainbow data processing program.

    Main Results:

    • Demonstrated that rainbow signal tilt angle correlates with droplet axial position, enabling 3D localization.
    • Achieved high accuracy in experimental validation with deionized water droplets.
    • Obtained refractive index with <0.0015 absolute error.
    • Measured droplet size with <±5% error.
    • Determined axial position with <±3% error.

    Conclusions:

    • Three-dimensional rainbow refractometry (TDRR) is a highly accurate and effective method for droplet characterization.
    • TDRR advances non-invasive droplet analysis by providing simultaneous 3D positional information.
    • The developed TDRR technique shows significant potential for various scientific and industrial applications.