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Related Experiment Video

Updated: Jun 22, 2025

Production and Purification of Baculovirus for Gene Therapy Application

Production and Purification of Baculovirus for Gene Therapy Application

Published on: April 9, 2018

Rapid Two-Day Baculovirus Titering Using Flow Cytometry.

Maya Yovcheva¹, Kenneth W Thompson²

¹Thermo Fisher Scientific, Frederick, MD, USA. Maya.Yovcheva@thermofisher.com.

Methods in Molecular Biology (Clifton, N.J.)

|July 1, 2024

Summary

This summary is machine-generated.

This study presents a fast method to quantify baculovirus stock using fluorescent antibody staining and flow cytometry. This technique accurately determines virus titers by analyzing infected insect cells within hours of inoculation.

Keywords:

Baculovirus Flow cytometry Titration

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A Method For Production of Recombinant mCD1d Protein in Insect Cells.

A Method For Production of Recombinant mCD1d Protein in Insect Cells.

Published on: December 10, 2007

Area of Science:

Biotechnology
Virology
Cell Biology

Background:

Accurate quantification of baculovirus stocks is crucial for reproducible results in insect cell culture and recombinant protein production.
Traditional methods for virus titration can be time-consuming and labor-intensive.

Purpose of the Study:

To develop and validate a rapid flow cytometry-based method for determining baculovirus titers.
To enable quick assessment of baculovirus stock concentration for experimental use.

Main Methods:

Infection of Spodoptera frugiperda (Sf9) insect cells with serial dilutions of baculovirus stock.
Staining of infected cells using a fluorescently labeled antibody targeting the viral gp64 envelope protein.
Quantification of infected cells using flow cytometry analysis.

Main Results:

The method allows for the quantitation of baculovirus-infected insect cells.
Titers are calculated based on the ratio of infected to uninfected cells.
Results are obtainable 13 to 18 hours post-inoculation.

Conclusions:

This rapid detection method provides an efficient way to determine baculovirus titers.
Flow cytometry-based quantitation offers a sensitive and high-throughput alternative to traditional titration methods.
The assay is suitable for routine use in baculovirus stock characterization.