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Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation
Published on: July 10, 2012
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Lobar lung transplantation in the mouse.
Jiang Shi1, Caikang Luo1, Yu Xu1
1Department of Thoracic Surgery and Oncology, State Key Laboratory and National Clinical Research Center for Respiratory Disease, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Journal of Thoracic Disease
|July 10, 2024
Summary
A new mouse model for lobar lung transplantation (LTx) was developed, improving donor organ utilization. This model shows comparable graft function and pathology to standard orthotopic LTx, offering a viable preclinical tool.
Area of Science:
- Surgical Innovation
- Transplantation Research
- Animal Models
Background:
- Clinical lobar lung transplantation (LTx) requires a stable animal model.
- Current orthotopic left LTx models discard the donor's right lung, leading to resource wastage.
- A novel mouse model for lobar LTx is needed to address these limitations.
Purpose of the Study:
- To introduce and validate a novel mouse model for lobar lung transplantation (LTx).
- To assess the feasibility and efficacy of this new lobar LTx model.
- To enhance the utilization rate of donor organs in experimental transplantation.
Main Methods:
- Performed lobar and orthotopic left LTx in syngeneic mouse strains.
- Assessed graft function using micro-computed tomography and arterial blood gases 28 days post-transplant.
- Evaluated pathological changes via Hematoxylin-eosin and Masson's trichrome staining.
Main Results:
- Achieved a 90% success rate for lobar LTx and 100% for orthotopic left LTx.
- Graft preparation time was longer for lobar LTx (42.11±3.79 min) vs. orthotopic left LTx (30.10±3.14 min; P<0.001).
- Graft function and histopathology were comparable between lobar and orthotopic left LTx at 28 days.
Conclusions:
- Successfully developed a mouse model for lobar lung transplantation (LTx).
- This model offers a potential preclinical platform for clinical lobar LTx.
- The novel model addresses resource wastage associated with current orthotopic left LTx techniques.
