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Comparative Assessment of miR-185-5p and miR-191-5p Expression: From Normal Endometrium to High-Grade Endometrial
Sergio Antonio Oropeza-de Lara1, Idalia Garza-Veloz1, Bertha Berthaud-González2
1Molecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km 6 Ejido la Escondida, Zacatecas 98160, Mexico.
Cells
|July 12, 2024
Summary
Two microRNAs, hsa-miR-185-5p and hsa-miR-191-5p, are underexpressed in endometrial cancer (EC) tissues. Their reduced levels correlate with disease grade, suggesting potential as diagnostic biomarkers and tumor suppressors in EC.
Area of Science:
- Oncology
- Molecular Biology
- Biomarkers
Background:
- Endometrial cancer (EC) is a leading cause of cancer mortality in women.
- MicroRNAs (miRs) are implicated in cancer development, functioning as oncogenes or tumor suppressors.
- Identifying novel biomarkers for EC diagnosis and understanding miR roles are crucial.
Purpose of the Study:
- To evaluate the diagnostic potential of hsa-miR-185-5p and hsa-miR-191-5p in endometrial cancer.
- To investigate the correlation between these miRs expression and clinical-histopathological features of EC.
- To explore the potential role of these miRs as tumor suppressors in EC development.
Main Methods:
- A cross-sectional study involving 59 patients with endometrial cancer, hyperplasia, normal endometrium, and polyps.
- Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) with TaqMan probes for miR expression analysis.
- Statistical analysis including Shapiro-Wilk test and parametric/non-parametric tests to assess miR expression correlations.
Main Results:
- Both hsa-miR-185-5p and hsa-miR-191-5p were found to be underexpressed in precursor and malignant endometrial lesions compared to normal or benign tissues.
- hsa-miR-185-5p underexpression was significant in grade 3 EC versus normal endometrium and hyperplasia subtypes.
- hsa-miR-191-5p underexpression was observed in hyperplasia and EC compared to secretory endometrium and polyps.
Conclusions:
- hsa-miR-185-5p and hsa-miR-191-5p are significantly underexpressed in endometrial cancer tissues.
- The expression levels of these miRs correlate with histopathological grades of EC.
- These miRs show promise as diagnostic biomarkers and tumor suppressors in endometrial cancer.
