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Assessment of Encapsulated Probiotic Lactococcus lactis A12 Viability Using an In Vitro Digestion Model for Tilapia.

Marcelo Fernando Valle Vargas1, María Ximena Quintanilla-Carvajal1, Luisa Villamil-Diaz1

  • 1Grupo de Investigación en Procesos Agroindustriales (GIPA), Doctorado en Biociencias, Facultad de Ingeniería, Universidad de La Sabana, Campus del Puente del Común, Km. 7, Autopista Norte de Bogotá, 250001 Chía, Cundinamarca, Colombia.

Animals : an Open Access Journal From MDPI
|July 13, 2024
PubMed
Summary

Encapsulated Lactococcus lactis A12 probiotics survived simulated fish gastrointestinal conditions. This in vitro model helps assess probiotic viability for aquaculture feed before live trials.

Keywords:
Lactococcus lactisNile tilapiadigestionencapsulatedin vitroprobiotic

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Area of Science:

  • Aquatic microbiology
  • Probiotics in aquaculture
  • Fish nutrition and health

Background:

  • Probiotics are crucial for fish health but must survive the harsh gastrointestinal tract (GIT).
  • Evaluating probiotic viability under simulated GIT conditions is essential before in vivo testing.
  • Encapsulation can protect probiotics during transit.

Purpose of the Study:

  • To assess the viability of encapsulated Lactococcus lactis A12 under simulated stomach and intestinal conditions in tilapia.
  • To evaluate the impact of pH, residence time, and enzyme concentration on probiotic survival.
  • To compare the efficacy of encapsulated versus non-encapsulated L. lactis A12.

Main Methods:

  • An in vitro digestion model simulating fish GIT conditions was employed.
  • A Box-Behnken design optimized the evaluation of stomach pH, residence time, and enzyme quantity.
  • Viability and antibacterial activity against Streptococcus agalactiae were measured.

Main Results:

  • Low pH (4.00), extended residence time (4 h), and high enzyme levels reduced final cell counts.
  • Encapsulated L. lactis A12 demonstrated significantly higher viability (p < 0.05) compared to non-encapsulated forms.
  • Encapsulated probiotics also exhibited enhanced antibacterial activity (p < 0.05) against S. agalactiae.

Conclusions:

  • Lactococcus lactis A12 shows resilience in simulated fish GIT environments.
  • Encapsulation significantly improves probiotic survival and efficacy in aquaculture.
  • The developed in vitro model is a valuable tool for screening probiotics for fish feed.