Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome?

Biomedicines·2026
Same author

Effects of Probiotic Supplementation on Gut Microbiota and Fecal Metabolome in Autism Spectrum Disorders: A Secondary Analysis of a Randomized Clinical Trial in Preschoolers.

Metabolites·2026
Same author

Advances in Thyroid Gland Regeneration: The Integrated Approach of Cell Biology and Bioengineering.

Tissue engineering. Part B, Reviews·2026
Same author

Bioartificial Cardiac Patches Functionalized with Apelin-13 Increase Cardiac C-Type Natriuretic Peptide Expression in Infarcted Rats.

Biomedicines·2026
Same author

Exploring <i>Castanea sativa</i> Shells (CSSs) as a Source of AKR1B1 and AKR1B10 Inhibitors: From Extraction to Bioactivity Testing.

Molecules (Basel, Switzerland)·2026
Same author

Identification of Connexin 26 on Extracellular Vesicles from Human Cardiomyocytes and Plasma: Novel Insights into miRNA Loading and Oxidative Injury.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Jun 21, 2025

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

2.0K

A Dynamic Cellular Model as an Emerging Platform to Reproduce the Complexity of Human Vascular Calcification In

Elisa Ceccherini1, Elisa Persiani1, Manuela Cabiati1

  • 1Institute of Clinical Physiology, National Research Council, 56124 Pisa, Italy.

International Journal of Molecular Sciences
|July 13, 2024
PubMed
Summary

A new dynamic co-culture model using a double-flow bioreactor effectively mimics vascular calcification (VC) in vivo. This advanced system reveals crucial microenvironment influences on VSMC calcification and inflammation, surpassing static monoculture limitations.

Keywords:
ECsVSMCsbioreactorsco-culturedynamic in vitro modelsvascular calcification

More Related Videos

Analysis of Extracellular Vesicle-Mediated Vascular Calcification Using In Vitro and In Vivo Models
09:01

Analysis of Extracellular Vesicle-Mediated Vascular Calcification Using In Vitro and In Vivo Models

Published on: January 27, 2023

1.6K
Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
08:43

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

19.5K

Related Experiment Videos

Last Updated: Jun 21, 2025

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

2.0K
Analysis of Extracellular Vesicle-Mediated Vascular Calcification Using In Vitro and In Vivo Models
09:01

Analysis of Extracellular Vesicle-Mediated Vascular Calcification Using In Vitro and In Vivo Models

Published on: January 27, 2023

1.6K
Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
08:43

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

19.5K

Area of Science:

  • Cardiovascular Biology
  • Biomedical Engineering
  • Cellular Pathophysiology

Background:

  • Vascular calcification (VC) involves calcium deposition in vascular smooth muscle cells (VSMCs).
  • Current in vitro VC models use static VSMC monocultures, lacking crucial cell interactions and dynamic conditions.
  • These limitations hinder the study of vascular pathophysiology.

Purpose of the Study:

  • To develop a dynamic endothelial cell-VSMC co-culture model.
  • To better replicate the in vivo vascular microenvironment for VC research.
  • To investigate VSMC calcification and inflammation under dynamic flow conditions.

Main Methods:

  • A double-flow bioreactor was utilized for endothelial cell-VSMC co-culture.
  • VSMC calcification was induced using high glucose and phosphate medium for 7 days.
  • Evaluated calcification, cell viability, inflammatory mediators, and molecular markers (SIRT-1, TGFβ1).

Main Results:

  • The dynamic co-culture model successfully reproduced VSMC calcification and inflammation.
  • Observed distinct modulation of effectors in the VSMC calcified phenotype compared to monocultures.
  • Highlighted the significant role of the microenvironment in controlling cellular behavior.

Conclusions:

  • The developed dynamic co-culture platform offers a more advanced system for VC research.
  • This model provides insights into VC pathophysiologic mechanisms not achievable with standard monocultures.
  • Emphasizes the importance of dynamic, multi-cellular interactions in understanding vascular disease.