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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
Published on: August 15, 2014
Visualization of Microcapillary Tips Using Waveguided Light
Chanbin Yoo1,2, Seung Kwon Seol1,2, Jaeyeon Pyo1,2
1Smart 3D Printing Research Team, Korea Electrotechnology Research Institute (KERI), Changwon 51543, Korea.
Abstract:
The microcapillary, a glass tube with a nano/micrometer scale aperture, is used for manipulating small objects across diverse disciplines. A primary concern in using the microcapillary involves tip breakage upon contact. Here, we report a method for visualizing the microcapillary tip, enabling precise and instant determination of its contact with other objects. Illumination directed to the back aperture of the microcapillary induces waveguiding through the glass wall, enabling the visualization of the tip through scattering. We demonstrate that the tip scattering is sensitive to contact with an adjacent object owing to the near-field interaction of the waveguided light, providing a clear distinction between the contact and noncontact states. The key advantage of our method encompasses its minimal influence, irrespective of conductivity, and applicability to nanoscale systems. The versatility of our method is shown by the application to a wide range of tip diameters, various substrate and in-filling materials.
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