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Nanobioactive Blood-Derived Shear-Thinning Biomaterial for Tissue Engineering Applications.
Ankit Gangrade1, Fatemeh Zehtabi1, Ahmad Rashad1
1Terasaki Institute for Biomedical Innovation (TIBI), 1018 Westwood Blvd, Los Angeles, California, USA.
Applied Materials Today
|July 15, 2024
Summary
This study introduces a novel injectable hydrogel for bone regeneration, using silicate nanoplatelets and plasma rich in growth factors (PRGF). The biomaterial shows promise for maxillofacial reconstruction due to its biocompatibility and bone-promoting properties.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Autografts for bone grafting face limitations like availability and donor site morbidity.
- Allografts and xenografts carry risks of rejection, highlighting the need for artificial bone graft alternatives.
- Injectable hydrogels offer a promising solution for bone regeneration, particularly in complex maxillofacial defects.
Purpose of the Study:
- To develop and evaluate a novel shear-thinning injectable hydrogel for maxillofacial bone regeneration.
- To assess the sustained release of proteins and growth factors from the hydrogel.
- To investigate the biocompatibility, hemostatic, angiogenic, and osteogenic potential of the developed hydrogel.
Main Methods:
- Formulation of an injectable hydrogel by blending silicate nanoplatelets (SNs) with plasma rich in growth factors (PRGF).
- Assessment of sustained protein and growth factor release kinetics from hydrogel formulations.
- In vitro and in vivo evaluation of hydrogel biocompatibility, hemostasis, angiogenesis, and osteogenic differentiation using assays like Alizarin Red staining and gene expression analysis.
Main Results:
- Hydrogel formulations with 7.5% PRGF demonstrated sustained release of proteins and growth factors.
- The hydrogel exhibited excellent in vitro and in vivo biocompatibility and hemostatic properties.
- Significant angiogenic potential and promotion of bone differentiation were observed, confirmed by biomarker assessments.
Conclusions:
- The developed shear-thinning hydrogel, enriched with PRGF, is a highly promising candidate for maxillofacial bone regeneration.
- This biomaterial offers precise control over growth factor release and demonstrates robust regenerative capabilities.
- The findings present a significant advancement in regenerative medicine, providing a platform for effective therapeutic strategies.
