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Updated: Jul 30, 2026
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A High-Throughput Platform for Culture and 3D Imaging of Organoids
Published on: October 14, 2022
2.7K
Recent advances in organoid engineering: A comprehensive review
Janitha M Unagolla1, Ambalangodage C Jayasuriya1,2
1Biomedical Engineering Program, Department of Bioengineering, College of Engineering, The University of Toledo, Toledo OH, United States.
Applied Materials Today
|January 24, 2024
Summary
Organoids are 3D tissue models that mimic human organs. Bioengineering techniques enhance organoid development for improved drug discovery and disease modeling, reducing animal testing.
Area of Science:
- Biotechnology and Bioengineering
- Stem Cell Biology
- Tissue Engineering
Background:
- Organoids are self-assembling 3D tissue constructs that recapitulate organ architecture and function in vitro.
- Advancements in stem cell culture and hydrogel extracellular matrices (ECM) enable organoid development.
- Organoids offer physiological relevance, reducing reliance on animal models.
Purpose of the Study:
- To review organoid formation and self-assembly principles.
- To discuss bioengineering approaches addressing limitations in traditional organoid culture.
- To highlight the role of hydrogel systems in stem cell microenvironments for organoid development.
Main Methods:
- Exploration of bioengineering technologies: microfluidics, bioreactors, 3D bioprinting, and organoids-on-a-chip.
- Discussion of natural and synthetic hydrogel systems.
- Review of selected organoid models and their applications.
Main Results:
- Bioengineering approaches overcome challenges in creating complex organoid microenvironments and cellular structures.
- Hydrogel systems are crucial for optimizing the stem cell microenvironment in organoid cultures.
- Organoid models demonstrate significant therapeutic applications in drug discovery and disease modeling.
Conclusions:
- Bioengineering significantly enhances the development and physiological relevance of organoids.
- Organoids hold great promise for advancing personalized medicine, drug screening, and understanding diseases.
- Further integration of bioengineering principles will drive innovation in organoid technology.
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